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Engulfment and cell motility protein 1 (ELMO1) is a cytoplasmic protein involved in the clearance of apoptotic cells by phagocytosis. ELMO1 is activated downstream of the phosphatidylserine receptor BAI1. ELMO1 binds to DOCK180 and facilitates its transport to the plasma membrane where DOCK180 activates the GTPase RAC1, possibly via Arf6. RAC1, like other small GTPases, mediate cytoskeleton remodeling and cell motility which is necessary for the internalization of target cells.
Product Information
Format
Affinity Purified
Control
Jurkat cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect ELMO1 using this Anti-ELMO1 Antibody validated for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human ELMO1 at the ELMO domain.
Epitope
ELMO domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes ELMO1 at the ELMO domain.
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Mouse, Bovine, and Rhesus Macaque based on 100% sequence homology. No homology to Rat was found.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_055615
Gene Symbol
ELMO1
Purification Method
Affinity Purfied
UniProt Number
Q92556
Molecular Weight
~78 kDa observed. This antibody exhibits strong affinity, high dilutions are recommended. The calculated molecular weight is 84 kDa, however ELMO1 has been shown as a ~78 kDa band in western blots (Gumienny, T.L., et al. (2001). Cell. 107:27–41.)
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ELMO1 on 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
