Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9714-I |
| Replaces | AB9714 |
| Description | Anti-RAGE |
| Alternate Names |
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| Background Information | Advanced glycosylation end product-specific receptor (UniProt: Q15109; also known as Receptor for advanced glycosylation end products, RAGE) is encoded by the AGER (also known as RAGE) gene (Gene ID: 177) in human. RAGE is a single-pass type I membrane protein that contains two Ig-like C2-type domains, one Ig-like V-type domain, a 21 amino acid transmembrane domain and a 41 amino acids cytoplasmic domain. RAGE mediates interactions of advanced glycosylation end products (AGE), which are non-enzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. RAGE acts as a mediator of both acute and chronic vascular inflammation in conditions, such as atherosclerosis and in complication of diabetes. Blockage of RAGE is reported to suppress development of atherosclerosis and vascular disorders. RAGE is expressed by endothelium, mononuclear phagocytes, neurons and smooth muscle cells. Higher expression of RAGE is observed during development, especially in the central nervous system, and its levels decline during maturity. Besides binding to advanced glycosylation end product (AGE), RAGE also binds amyloid beta peptide, S100family proteins, and leukocyte integrins. Excessive amounts of amyloid peptides (Ab) in brain leads to the hyperstimulation of RAGE. The RAGE-Ab interaction is thought to result in oxidative stress leading to neuronal degeneration. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RAGE, Cat. No. AB9714-I, is a highly specific rabbit polyclonal antibody that targets Advanced glycosylation end product-specific receptor and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAGE in human lung tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-cojugated linear peptide corresponding to 20 amino acids from the N-terminal region of human RAGE. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects Receptor for Advanced Glycosylation End Products (RAGE). This antibody targets an epitope corresponding to 21 amino acids in the N-terminal region of human RAGE. Human, mouse, and rat RAGE display 95% homology in this epitope region. |
| Species Reactivity |
|
| Species Reactivity Note | Rat, Mouse. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~48 kDa observed; 42.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse lung tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected RAGE in 10 µg of mouse lung tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |