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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ICC, IHC, IH(P), IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9610 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Olig-2 Antibody |
| Alternate Names |
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| Background Information | Oligodendrocyte transcription factor 2 (UniProt: Q13516, also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is expressed in the brain in oligodendrocytes. Its strong expression is also observed in oligodendrogliomas. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It functions together with ZNF488 to promote oligodendrocyte differentiation. Olig2 is observed in both nucleus and the cytoplasm. Its nuclear localization signal is contained in the bHLH domain (aa 108-162). It can be masked in the native form and translocation to the nucleus may be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Olig2 cooperates with Olig1 to establish the motor neuron progenitor domain of the embryonic neural tube. Removal of Cdx transcription factors is shown to result in the continued induction of Olig2 and ectopic production of cranial motor neuron progenitors. A chromosomal aberration involving Olig2 is shown to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Metzis, V., et al. (2018). Cell 175(4); 1105-1118). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord). Immunoprecipitation: 1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line. Optimal working dilutions must be determined by the end user. Immunocytochemistry: 1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord). Optimal working dilutions must be determined by the end user |
| Biological Information | |
|---|---|
| Immunogen | Recombinant mouse Olig-2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes the ~32 kDa Olig-2 protein by Western blot. |
| Species Reactivity |
|
| Species Reactivity Note | Human, rat and mouse. Other species have not been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. |
| Gene Symbol |
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| Purification Method | Ammonium Sulfate Precipitation |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13516 # Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity). SIZE: 323 amino acids; 32385 Da SUBUNIT: Interacts with NKX2-2 (By similarity). SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2 (By similarity). TISSUE SPECIFICITY: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. DOMAIN:SwissProt: Q13516 The bHLH is essential for interaction with NKX2-2 (By similarity).DISEASE:SwissProt: Q13516 # A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. ... |
| Molecular Weight | ~32 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by immunohistochemistry on glioblastoma Immunohistochemistry (Paraffin): Representative lot data. Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB. Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |