Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB939-I |
| Description | Anti-Pancreatic Polypeptide |
| Alternate Names |
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| Background Information | Pancreatic polypeptide (UniProt: P01298; also known as PP, Obinepitide, Pancreatic prohormone) is encoded by the PPY (also known as PNP) gene (Gene ID: 5539) in human. Pancreatic polypeptide is a member of the neuropeptide Y family and is secreted protein that is produced by PP cells in the pancreatic islets of Langerhans. It is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off. It acts as a regulator of pancreatic and gastrointestinal functions. Two isoforms of pancreatic polypeptide have been described that are generated by alternative splicing. Pancreatic polypeptide release is functionally associated with increased satiety, delayed gastric emptying, inhibition of gallbladder motility, and regulation of energy expenditure. Its deficiency is observed in cases of pancreatic resection and chronic pancreatitis and is associated with significant reduction in PP cell mass and abnormally high hepatic glucose production even in the presence of endogenous insulin. (Ref.: Maxwell, J et al. (2014). Pancreas 43(4); 651-656). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Pancreatic Polypeptide Antibody, Cat. No. AB939-I, is a highly specific rabbit polyclonal antibody that targets Pancreatic polypeptide and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1:2,000 dilution from a representative lot detected Pancreatic Polypeptide in 10 µg of human pancreas tissue lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections. Immunocytochemistry Analysis (ICC): A 1:500 dilution from a representative lot detected Pancreatic Polypeptide in TGP52 cell line. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 36 amino acids the internal region of human pancreatic polypeptide. |
| Epitope | cytoplasmic domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects both isoforms of human pancreatic polypeptide. It targets an epitope within 36 amino acids from the internal region. |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~11 and ~20 kDa observed for isoforms 1 and 2; 10.45 and 18.79 kDa calculated for isoforms 1 and 2. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PANC-1 cell lysate. Western Blotting Analysis: 1:2,000 dilution of this antibody detected Pancreatic Polypeptide in 10 µg of PANC-1 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |