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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9272 |
| Replaces | 04-1019 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Synaptophysin Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 containing BSA and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Synaptophysin Antibody detects level of Synaptophysin & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Western blot Immunohistochemistry on formalin fixed, paraffin embedded tissue: 1:300 for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Optimal working dilutions must be determined by the end user. STAINING PATTERN: Cytoplasmic |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from human synaptophysin. |
| Host | Rabbit |
| Specificity | Synaptophysin |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. SIZE: 313 amino acids; 33845 Da SUBUNIT: Homohexamer or homotetramer. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity). SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C for up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |