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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9240 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-NeuroD 6 Antibody |
| Overview | To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-NeuroD 6 Antibody is validated for use in WB, IH for the detection of NeuroD 6. |
| Key Applications |
|
| Application Notes | Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide. |
| Host | Rabbit |
| Specificity | Recognizes NeuroD 6 (MATH2, NEX1), a Helix-loop-helix class of transcription factor. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | NEUROD6 is a member of the NEUROD (NEUROD1; MIM 601724) family of basic helix-loop-helix (bHLH) transcription factors (Guo et al., 2002 [PubMed 12357074]).[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q96NK8 # Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene (By similarity). SIZE: 337 amino acids; 38705 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein (By similarity). SUBCELLULAR LOCATION: Nucleus (Probable). SIMILARITY: SwissProt: Q96NK8 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain unopened vial at -70C° for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |