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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB81023 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Calpain II Antibody, large subunit |
| Alternate Names |
|
| Background Information | Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS pH 7.4 containing 1% BSA and 50% glycerol as a stabilizer. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Calpain II Antibody, large subunit is an antibody against Calpain II for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1000 for colorimetric substrates or 1:5000 for chemiluminescent substrates (recommended starting concentration). Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Epitope | large subunit |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The anti-calpain II antibody recognizes the 80 kDa and 58 kDa forms of Calpain II. It does not cross-react with other calpains. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5 UTR have been reported. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P17655 # Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. COFACTOR: Binds 3 calcium ions. SIZE: 700 amino acids; 80009 Da SUBUNIT: Forms a heterodimer with a small (regulatory) subunit (CAPNS1). SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: SwissProt: P17655 ## Belongs to the peptidase C2 family. & Contains 1 calpain catalytic domain. & Contains 3 EF-hand domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20*C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µl |