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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6019 |
| Description | Anti-Thymosin beta-4 Antibody |
| Alternate Names |
|
| Background Information | Thymosin beta-4 is the most prevalent of the cytoplasmic G-actin binding proteins belonging to the highly conserved thymosin beta family. Known to bind actin monomers, Thymosin beta-4 sequesters G-actin in a manner that prevents actin polymerization. Thymosin beta-4 plays several important roles including induction of tissue remodeling, wound healing in both cell and tissue injuries, and cell differentiation via mechanisms that have yet to be fully elucidated. It is involved in the regulation of the cytoskeleton, and thereby influences an array of cellular processes. Within the brain, Thymosin beta-4 is associated with the neurite extension and migration of neurons. Expression has been observed in various hemopoietic and lymphoid malignant cell lines. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Thymosin beta-4 Antibody is an antibody against Thymosin beta-4 for use in WB & IC. |
| Key Applications |
|
| Application Notes | Western Blot (Snap i.d.®) Analysis: 1 µg/mL from a previous lot detected Thymosin beta-4 on 10 µg of recombinant Thymosin beta-4. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Thymosin beta-4 in NIH/3T3, HeLa, and A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the internal domain of human Thymosin beta-4. |
| Epitope | Unknown |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Th antibody recognizes the internal domain of Thymosin beta-4. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human and mouse. Predicted to react with rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays an important role in the organization of the cytoskeleton (by similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase (by similarity). SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. TISSUE SPECIFICTY: Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells. INDUCTION: By alpha interferons. Decreased levels in THP-1 cells after treatment with recombinant interferon-lambda. SEQUENCE SIMILARITIES: Belongs to the thymosin beta family. |
| Molecular Weight | ~ 33kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in recombinant Thymosin beta-4. Western Blot Analysis: 1 µg/mL of the antibody detected Thymosin beta-4 on 10 µg of recombinant Thymosin beta-4. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |