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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ca, Pm, B | WB, ICC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6017 |
| Description | Anti-F-actin-capping protein subunit beta Antibody |
| Alternate Names |
|
| Background Information | F-actin-capping-protein subunit beta (CapZ beta) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. F-actin capping protein subunit beta is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of F-actin capping protein subunit beta’s subunits. While there is almost nothing known regarding the β3 subunit, the β1 and β2 isoforms each have distinct roles. The isoform β2 is predominantly expressed in non-muscle cells and is found in intercalated discs and the cell periphery, but is not observed at Z-discs. Isoform β1 is more greatly expressed in striated muscle and is localized at the Z-discs. It also contains the COOH-terminal extension necessary for capping the actin. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect F-actin-capping protein subunit beta using this Anti-F-actin-capping protein subunit beta Antibody validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect F-actin-capping protein subunit beta in NIH/3T3 and A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human F-actin-capping protein subunit beta of the C-terminus of isoform 2. |
| Epitope | C-terminus of Isoform 2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes F-actin-capping protein subunit beta at the C-terminus of isoform 2. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human, mouse, and rat. Predicted to react with canine, primate, and bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | CAPZB is a member of the F-actin capping protein family. This gene encodes the beta subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. SUBUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. Interacts with ARHGAP17 and RCSD1/CAPZIP. Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton (By similarity). SEQUENCE SIMILARITIES: Belongs to the F-actin-capping protein beta subunit family. |
| Molecular Weight | ~ 34 kDa observed MW. There are 3 isoforms produced by alternative splicing: Isoform 1 at 31 kDa, Isoform 2 at 31 kDa, and Isoform 3 (sequence not available). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit beta in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |