Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5840-I |
| Description | Anti-Dab1 |
| Alternate Names |
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| Background Information | Disabled homolog 1 (UniProt: P97318; also known as DAB1) is encoded by the Dab1 gene (Gene ID: 13131) in murine species. Dab 1 is mainly expressed in the brain and serves as an adapter molecule involved in neural development where it is reported to direct the migration of cortical neurons past previously formed neurons to reach their proper layer. DAB1 is can be phosphorylated on Tyr198, Tyr220, and Tyr232 upon reelin induction in embryonic neurons. It can also be phosphorylated on Ser524, but this phosphorylation is independent of reelin signaling. Tyrosine phosphorylation of DAB1 by Src family tyrosine kinases is considered to be the most critical downstream event in Reelin signaling. DAB1 contains a phosphotyrosine interaction domain (PID) (aa 36-189), which specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins. Seven different isoforms of Dab1 have been identified that are generated via alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Dab1, Cat. No. AB5840-I, is a highly specific rabbit polyclonal antibody that targets Disabled homolog 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Dab1 in mouse cerebral cortex tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 156 amino acids from the C-terminal half of murine Disabled homolog 1 (Dab1). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects murine Disabled homolog 1 (Dab1). It targets an epitope within 156 amino acids from the C-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~64 kDa observed; 63.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Dab1 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |