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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5727 |
| Replaces | AB5774 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Sox10 Antibody |
| Background Information | Sox 10 is part of a family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Sox 10 is expressed in multipotent neural crest stem cells, and its expression is downregulated upon their differentiation. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sox10 Antibody detects level of Sox10 & has been published & validated for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 0.1-0.5 μg/mL using ECL on fetal mouse brain tissue lysate. The antibody reacts with proteins of ~60 kDa. An additional band of ~120 kDa may also be seen depending on sample used. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from Sox10. |
| Host | Rabbit |
| Specificity | Sox10. The immunogen sequence is 70% conserved with Sox8 and Sox9 and therefore the antibody may show cross reactivity to these proteins. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P56693 # Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia (By similarity). SIZE: 466 amino acids; 49911 Da SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Expressed in fetal brain and in adult brain, heart, small intestine and colon. DISEASE: SwissProt: P56693 # Defects in SOX10 are a cause of Waardenburg syndrome type IV (WS4) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4 is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). & Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome [MIM:601706]. The disorder consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome. & Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. SIMILARITY: SwissProt: P56693 ## Contains 1 HMG box DNA-binding domain. |
| Molecular Weight | Approx. 55 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at +2-8°C in undiluted aliquots for up to 6 months after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |