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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ICC, IF | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5675-AF488 |
| Description | Anti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488 |
| Alternate Names |
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| Background Information | Metabotropic glutamate receptor 5 or mGluR5 (UniProt P31424; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5) gene (Gene ID 24418) in rat species. The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488 is an antibody against Metabotropic Glutamate Receptor 5 for use in Immunocytochemistry, Immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: An 1:100 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in rat cortical neurons. The unconjugated antibody (Cat. No. AB5675) is shown to be suitable also for Western blotting and immunohistochemistry applications. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to a sequence from the C-terminal cytoplasmic domain of rat mGluR5. |
| Epitope | C-terminal cyoplasmic domain. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with all spliced isoforms of human, rat, and mouse mGluR5 reported by UniProt (P41594, P31424 and Q3UVX5, respectively). |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Predicted to react with Mouse, Human, Bovine, Primate, Feline, Horse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunofluorescence in rat brain tissue sections. Immunofluorescence Analysis: An 1:100 dilution of this antibody detected Metabotropic Glutamate Receptor 5 in rat cerebellum and rat brain tissue sections. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |