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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, M, R | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5652P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Histamine Receptor 1 Antibody, CT |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Histamine Receptor 1 Antibody, C-terminus is validated for use in ELISA, WB for the detection of Histamine Receptor 1. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. Recognizes a 57kDa band representing the H1 receptor ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 17 amino acid peptide sequence near the cytoplasmic, C-terminus of rat HR1 (Fujimoto et al. 1993; Inoue et al. 1996; DeBacker et al. 1993; Fukui et al. 1994; Moguilevsky et al. 1994). |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat Histamine Receptor 1 (HR1). The immunogen shows no significant sequence homology with other histamine receptors. The immunogen peptide is 100% conserved in mouse and bovine, 94% in human, fish and monkey and 80% in guinea pig. Reactivity with other species has not been confirmed. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Gene Symbol |
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| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P35367 # In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. SIZE: 487 amino acids; 55784 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule. SIMILARITY: SwissProt: P35367 ## Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |