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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5574-50UL |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-TrpC6 Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TrpC6 Antibody is an antibody against TrpC6 for use in IH & WB. |
| Key Applications |
|
| Application Notes | Western blot:1:200-1:400 using ECL on rat brain membranes. Immunohistochemstry on mouse brain sections. Dilutions should be made using a carrier protein such as BSA (1-3%) Addition of 0.1-0.5% Tween-20 to the antibody solution is recommended. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Peptide corresponding to amino acids 24-38 from mouse TRPC6 (Zhu et al. 1996). Accession Q61143 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes TRPC6 (Transient Receptor Potential-Like Channel 6, TRP-6). Does not cross react with any other known proteins. SPECIES REACTIVITIES: It is expected that the antibody will also work on human due to sequence conservation (15/16). Other species have not been tested. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9Y210 # Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. SIZE: 931 amino acids; 106326 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). TISSUE SPECIFICITY: Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. DISEASE: SwissProt: Q9Y210 # Defects in TRPC6 are the cause of focal segmental glomerulosclerosis 2 (FSGS2) [MIM:603965]. FSGS2 is an autosomal dominant disease characterized by increased urinary protein excretion (proteinuria) and decreasing kidney function (nephrotic syndrome). Renal insufficiency often progresses to end-stage renal disease (ESRD) (also known as end-stage renal failure), a highly morbid state requiring either dialysis therapy or kidney transplantation. SIMILARITY: SwissProt: Q9Y210 ## Belongs to the transient receptor family. STrpC subfamily. & Contains 4 ANK repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |