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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC | Gp | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5519 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Prodynorphin Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:500 Optimal working dilutions must be determined by the end user. APPLICATION NOTES FOR AB5519 IMMUNOHISTOCHEMISTRY Perfusion: 1) Ca2+-free Tyrodes solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from rat ProDynorphin. |
| Host | Guinea Pig |
| Specificity | ProDynorphin, rat. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01213 # Leumorphin has a typical opiod activity and may have anti-apoptotic effect (By similarity). SIZE: 254 amino acids; 28385 Da SUBCELLULAR LOCATION: Secreted. PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. SIMILARITY: SwissProt: P01213 ## Belongs to the opioid neuropeptide precursor family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |