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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5405 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Opsin Antibody, Red/Green |
| Background Information | Long-wave-sensitive opsin 1/Medium-wave-sensitive opsin 1 (UniProt: P04000/P04001; also known as Red cone photoreceptor pigment/Green cone photoreceptor pigment, Red-sensitive opsin/ Green-sensitive opsin, ROP/GOP) are encoded by the OPN1LW/OPN1MW (also known as RCP/GCP) genes (Gene ID: 5956/2652) in human. The full range of color discrimination in humans is based on the presence and function of three cone photoreceptors. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein that gives sensitivity in the short (S cone, peak sensitivity about 420 nm), middle (M cone, peak sensitivity about 530 nm with polymorphism), and long (L cone, peak sensitivity about 560 nm with polymorphism) wavelengths of the light spectrum. Opsins are multi-pass membrane proteins that belongs to the G-protein coupled receptor 1 family. They consist of four extracellular, 7 helical, and four cytoplasmic domains. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event. The S cone (blue) opsin seems to have a stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments. Mutations in OPN1MW and OPN1LW genes are known to cause color blindness that is characterized by a dichromasy in which red and green are confused, without loss of luminance or shift or shortening of the spectrum. Some mutations also lead to cone dystrophy leading to progressive degeneration of the cone photoreceptor with some preservation of rod function. (Ref.: Neitz, M., and Neitz, J. (2000). Arch. Ophthalmol. 118(5); 691-700). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Opsin Red/Green, Cat. No. AB5405, is a rabbit polyclonal antibody that detects Opsin Red/Green and is tested for use in Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Full-length, recombinant human red/green opsin. |
| Epitope | Red/Green |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Red-sensitive opsin/ Green-sensitive opsins. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Reactivity with other species has not been determined |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called red cone photopigment or long-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. This gene and the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of partial, protanopic colorblindness. |
| Gene Symbol |
|
| Purification Method | Protein A chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P04000 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. SIZE: 364 amino acids; 40572 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells. PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. DISEASE:SwissProt: P04000 # Defects in OPN1LW are the cause of partial colorblindness protan series (CBP) [MIM:303900]; also called protanopia.SIMILARITY:SwissProt: P04000 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in Mouse retina tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Opsin Red/Green in Mouse retina tissue sections. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |