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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5384 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Nitric Oxide Synthase II Antibody |
| Alternate Names |
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| Background Information | The inducible isoform of the enzyme nitric oxide synthase (iNOS, NOS-II) is synthesized by macrophages and many other cell types in response to proinflammatory cytokines and certain other stimuli, such as hypoxia and stress. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit serum. Liquid containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Nitric Oxide Synthase II using this Anti-Nitric Oxide Synthase II Antibody validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000-1:2,500 (ECL). Immunohistochemistry: 1:2,500-1:6,000 (ABC). Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | C-terminal 19 amino acid peptide from human chondrocyte nitric oxide synthase. |
| Host | Rabbit |
| Specificity | Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with nNOS and eNOS by immunohistochemistry. AB5384 reacts with human glia and certain neurons in Alzheimers disease by immunohistochemistry. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity). SIZE: 1153 amino acids; 131117 Da SUBUNIT: Homodimer. Binds SLC9A3R1. TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |