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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ELISA, WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5338P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cocaine and Amphetamine Related Transcript Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. No preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Cocaine & Amphetamine Related Transcript Antibody is validated for use in ELISA, WB, IH for the detection of Cocaine & Amphetamine Related Transcript. |
| Key Applications |
|
| Application Notes | Western blotting: 1-10 μg/mL using ECL. Immunohistochemistry: 5-10 μg/mL It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus. ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide (Catalog Number AG228) per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Mouse/rat CART peptide (55-102). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes Cocaine- and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | CART appears to have roles in reward, feeding, and stress, and it has the functional properties of an endogenous psychostimulant (Kuhar et al., 2002 [PubMed 12147208]).[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q16568 # Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. SIZE: 116 amino acids; 12829 Da SUBCELLULAR LOCATION: Secreted (Potential). TISSUE SPECIFICITY: Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus. DISEASE:SwissProt: Q16568 # A defect in CART is associated with reduced resting energy expenditure and cosegregates with obesity phenotype. SIMILARITY: SwissProt: Q16568 ## Belongs to the CART family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |