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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H, Mk | IHC, ICC | Rb | Biotin | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5320B |
| Description | Anti-NG2 Chondroitin Sulfate Proteoglycan, Biotin Conjugate Antibody |
| Alternate Names |
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| Background Information | NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor. |
| Product Information | |
|---|---|
| Format | Biotin |
| Control |
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| Presentation | Purified rabbit polyclonal conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NG2 Chondroitin Sulfate Proteoglycan, Biotin Conjugate Antibody is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in IHC, ICC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in paraffin embedded rat cortex tissue. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton. |
| Biological Information | |
|---|---|
| Immunogen | Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat |
| Host | Rabbit |
| Specificity | This antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Rat. Predicted to react with Mouse, Human, and Monkey based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. SIZE: 2322 amino acids; 250497 Da SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16. SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity). TISSUE SPECIFICITY: Detected only in malignant melanoma cells. PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity). SIMILARITY: Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains. MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells |
| Molecular Weight | The unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in cryosections of rat cortex tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in cryosections of rat cortex tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |