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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, Sh | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5118 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cyclooxygenase-2 Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Cyclooxygenase-2 Antibody is validated for use in ELISA, WB for the detection of Cyclooxygenase-2. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using Chemiluminescence technique; COX-2 runs as a 68-72KDa protein in most cells, sometimes a 90kDa post-translationally modified form is seen as well. CaCo-2 line extracts (induced with serum or growth factors) are good positive controls. ELISA: 1:10,000 - 1:100,000 using 100 ng of immunogen peptide per well. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | 17 amino acid synthetic peptide corresponding to a sequence near the C-terminus of Human/Mouse/Rat COX-2 coupled to KLH. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Cyclooxygenase-2. Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 and COX2. Both forms of COX proteins are membrane associated heme proteins containing cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin. COX2 is a 72 kDa protein having 60% homology to COX1. It is induced by cytokines and mitogens and likely plays a role in inflammatory diseases such as rheumatoid arthritis. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS2, which shows 86% - 89% amino acid sequence identity with mouse, rat, sheep, bovine, horse and rabit PTGS2 proteins, respectively. Human PTGS2 is expressed in a limited number of cell types and regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. The expression of this gene is deregulated in epithelial tumors. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P35354 # May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group per subunit (By similarity). SIZE: 604 amino acids; 68996 Da SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein. DISEASE:SwissProt: P35354 # Likely to play a role in inflammatory diseases such as rheumatoid arthritis. SIMILARITY: SwissProt: P35354 ## Belongs to the prostaglandin G/H synthase family. & Contains 1 EGF-like domain. MISCELLANEOUS: This enzyme acts both as a dioxygenase and as a peroxidase. & This enzyme is the target of nonsteroidal anti- inflammatory drugs such as aspirin. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |