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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Mk | ELISA, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5084P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Dopamine D2 Receptor Antibody |
| Alternate Names |
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| Background Information | The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal liquid in PBS with 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Dopamine D2 Receptor Antibody is validated for use in ELISA, IH, IP, WB for the detection of Dopamine D2 Receptor. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands. Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections on a previous lot. Immunohistochemistry: 1-20 µg/mL on paraformaldehyde fixed sections. Immunoprecipitation: 1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2). ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic loop #3. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Specific for Dopamine Receptor D2 short and long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (2). The immunogen peptide shows no significant homology with other dopamine receptors (D1 and D3-D5). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P14416 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. SIZE: 443 amino acids; 50619 Da SUBUNIT: Interacts with GPRASP1, neurabin-2 and CLIC6 (By similarity). Interacts with CADPS and CADPS2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. DISEASE: SwissProt: P14416 # It has been suggested that DRD2 is involved in psychiatric disorders; especially in schizophrenia. & Defects in DRD2 are associated with myoclonus dystonia (MD) [MIM:159900]; also known as myoclonic dystonia, alcohol- responsive dystonia and dystonia-11 (DYT11). MD is a movement disorder characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. SIMILARITY: SwissProt: P14416 ## Belongs to the G-protein coupled receptor 1 family |
| Molecular Weight | 50 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on human brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 μg of human brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |