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Millipore Detect Choline Acetyltransferase Using This Anti-Choline Acetyltransferase Antibody Validated For Use In Elisa, Ih. - Mill (Additional S&H or Hazmat Fees May Apply)
Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 μL of sterile distilled water. Centrifure to remove any insoluble material.
Quality Level
MQ100
Applications
Application
Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in ELISA, IH.
Key Applications
ELISA
Immunohistochemistry
Application Notes
Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, mouse primate and human ChAT.
Host
Rabbit
Specificity
Choline acetyltranferase. The antibody will stain cholinergic neurons in the rat central and peripheral nervous systems.
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_020549.3
NM_020984.2
NM_020985.2
NM_020986.2
Entrez Gene Summary
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5 untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
Gene Symbol
CHAT
ChAT
CMS1A2
CHOACTase
CMS1A
EC 2.3.1.6
UniProt Number
P28329
UniProt Summary
FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain lyophilized material dry at -70°C for up to 12 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
