Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB4235 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-AMACR Antibody |
| Alternate Names |
|
| Background Information | Prostate-specific antigen (PSA) screening for prostate cancer is now widespread in the United States among men of all ages. However PSA has limited specificity because benign disease, including prostatic enlargement and inflammation, can increase PSA levels. Thus, a more specific prostate cancer markers is needed. One such potential marker is AMACR, an enzyme that is involved in peroxisomal beta-oxidation of dietary branched-chain fatty acids. Recent studies have shown that, compared with expression in normal or benign prostate epithelium, AMACR is consistently overexpressed in prostate cancer epithelium, making it a specific marker for cancer cells within the prostate gland. Furthermore, overexpression of AMACR may increase the risk of prostate cancer because its expression is increased in premalignant lesions (prostatic intraepithelial neoplasia). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Peptide affinity purified antibody. Liquid in PBS, with 0.02% sodium azide as a preservative. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Detect AMACR using this Anti-AMACR Antibody validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Western blot: 1:500 Immunohistochemistry: 1:100 in an ABC method. An incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic human AMACR peptide. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes human AMACR (alpha-methylacyl-CoA racemase). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Alpha-methylacyl-CoA racemase is responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers, which are the only stereoisomers that can be degraded via peroxisomal beta-oxidation.[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9UHK6 # Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. SIZE: 382 amino acids; 42360 Da SUBCELLULAR LOCATION: Peroxisome. Mitochondrion. DISEASE: SwissProt: Q9UHK6 # Defects in AMACR are the cause of AMACR deficiency [MIM:604489]. It results in elevated concentrations of pristanic acid. It is associated with a form of adult onset sensory motor neuropathy. & Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile. SIMILARITY: SwissProt: Q9UHK6 ## Belongs to the caiB/baiF CoA-transferase family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8ºC in undiluted aliquots for 3 months or at -20°C for up to one year from date of receipt. Avoid repeated freeze-thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |