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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3738-I |
| Replaces | AB3738 |
| Description | Anti-Beta Lactamase |
| Alternate Names |
|
| Background Information | Beta-lactamase TEM (EC 3.5.2.6; UniProt P62593; also known as IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2) is encoded by the bla (also known as blaT-3, blaT-4, blaT-5, blaT-6) gene (Gene ID 3244915) in E. coli. TEM-1 belongs to the class-A beta-lactamase family. Beta-lactamases produced by bacteria provide resistance to beta-lactam antibiotics, such as penicillins and cephamycins by breaking open the beta-lactam ring. Over 200 beta-lactamases have been reported that are classified into four main groups (A, B, C, and D) and eight subgroups according to their functional and structural characteristics. The TEM-1 is predominant plasmid-mediated beta-lactamase of gram-negative rods. Six different nucleotide sequences, called blaTEM-1a, blaTEM-1b, blaTEM-1c, blaTEM-1d, blaTEM-1e, and blaTEM-1f, have been described to codify the same TEM-1 beta-lactamase. Ref.: Briñas, L., et al. (2002). Antimicrob. Agents Chemother. 46, 103156-103163. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect beta-lactamase using this rabbit Anti-Beta Lactamase polyclonal antibody, Cat. No. AB3738-I, validated for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full-length E. coli beta-lactamase, including the signal peptide sequence. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects E. coli beta-lactamase. |
| Species Reactivity |
|
| Species Reactivity Note | E. Coli. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
|
| Molecular Weight | 31.52/28.91 kDa (with/without signal peptide a.a. 1-23) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in lasate from beta-lactamase-transformed E.coli. Western Blotting Analysis: A 1:100,000 dilution of this antibody detected GST-tagged full-length E. coli beta-lactamase in 0.5 µg of lysate from transformed E. coli. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |