Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3540P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Monocarboxylate Transporter 1 Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Liquid in PBS containing 0.1% BSA . |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Monocarboxylate Transporter 1 using this Anti-Monocarboxylate Transporter 1 Antibody validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-5 μg/mL using ECL. Membrane preps are recommended. ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 17 amino acid peptide sequence near the C-terminus of rat MCT1 (Gerhart et al. 1997; Knott et al. 1999; Takanaga et al. 1995; Carpenter et al. 1996; Koehler-Stec et al. 1998; Yoon et al. 1999; Halestrap & Price 1999). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes rat Monocarboxylate Transporter 1 (MCT1). The immunogen shows no significant sequence homology with MCT2-8. SPECIES REACTIVITIES: The immunogen sequence is 93% conserved in hamster, 80% in mouse and only 40% in human. Reactivity with other species has not been confirmed. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P53985 # Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. SIZE: 500 amino acids; 53958 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed in normal and in cancer cells. DISEASE: SwissProt: P53985 # Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT) [MIM:245340]; also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals. SIMILARITY: SwissProt: P53985 ## Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |