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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, ICC, IF, IHC, IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3242 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-PGC-1 Antibody |
| Alternate Names |
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| Background Information | PGC-1 is a transcription factor that determines whether preadipocytes will differentiate into brown or white fat cells. It interacts with PPARgamma to promote the expression of a cassette of genes which define the brown adipocyte phenotype. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 20 mM PBS and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PGC-1 Antibody is a rabbit olyclponal antibody for the detection of PGC-1 also known as PPARgamma Coactivator 1-alpha & has been validated in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | ELISA Analysis: A 1:75,000 dilution of a previous lot was used in ELISA. Immunocytochemistry Analysis: A 1:300 dilution of a previous lot was used in Immunocytochemistry. Immunofluorescence Analysis: Representative lots detected PGC-1α in frozen mouse and rat muscle tissue sections (Gouspillou, G., et al. (2013). Longev. Healthspan. 2(1):13; Matsuura, T., et al. (2007). J. Orthop. Res. 25(11):1534-1540). Immunohistochemistry Analysis: A representative lot detected PGC-1α in free-floating mouse forebrain sections (Tallaksen-Greene, S.J., et al. (2014). J Neurosci. 34(47):15658-15668). Immunoprecipitation Analysis: Representative lots immunoprecipitated PGC-1α from mouse liver, skeletal muscle, and brown adipose tissue lysates (Boutant, M., et al. (2016). Cell Rep. 14(9):2068-2075; Park, R.D., et al. (2014). J. Exerc. Nutrition Biochem. 18(1):1-7; Kim, J.S., et al. (2014). J. Exerc. Nutrition Biochem. 18(3):259-266; Philip, A., et al. (2011). J. Biol. Chem. 286(35):30561-30570). Western Blotting Analysis: Representative lots detected similar PGC-1α in human, mouse, and rat skeletal muscle tissue, as well as in nuclear and mitochondrial preparations from rat brown adipose tissue (Suwa, M., et al. (2015). J. Sports Sci. Med. 14(3):548-555; Lee, I., et al. (2015). Front. Pharmacol. 6:43; Vincent, G., et al. (2015). Front Physiol. 6:51; Lombardi, A., et al. (2015). PLoS One. 10(2):e0116498; Gouspillou, G., et al. (2014). FASEB J. 28(4):1621-1633; Saleem, A., et al. (2014). Am. J. Physiol. Cell Physiol. 306(3):C241-249). |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from amino acids 777-797 (C-terminus) of mouse PGC-1. |
| Epitope | C-terminus |
| Host | Rabbit |
| Specificity | Immunogen peptide blocking prior to Western blotting completely abolished target band detection by this rabbit polyclonal antibody. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. |
| Gene Symbol |
|
| Purification Method | Affinity purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. SIZE: 797 amino acids; 90,588 Da SUBUNIT: Binds MYBBP1A, which inhibits transcriptional activation by this protein (By similarity). Interacts with LRPPRC. Homooligomer. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Brown adipose tissue, heart, kidney and brain. SIMILARITY: Contains 1 RRM (RNA recognition motif) domain. |
| Molecular Weight | ~100 kDa observed. 91.03/89.64/91.71/77.14 kDa (human isoform 1/B4/B5/L-PGG-1alpha), 90.59 kDa (mouse isoform 1; PGC-1a1), 90.62 kDa (rat) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse BAT extracts. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant mouse PGC-1, as well as endogenous PGC-1 in 15 µg of nuclear extract and whole cell lysate from mouse brown adipose tissue (BAT). Immunogen peptide blocking completely blocked target band detection. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -70ºC from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |