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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3241-25UL |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Cdc2 Antibody, phospho-specific (Tyr15) |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Cdc2 Antibody, phospho-specific (Tyr15) (Rabbit Polyclonal Antibody) validated in WB to detect Cdc2 also known as Cell Division Cycle 2, p34 Protein Kinase. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids surrounding the phosphoTry15 of human, rat, mouse and Xenopus CDC2. |
| Epitope | phospho-specific (Tyr15) |
| Host | Rabbit |
| Specificity | CDC2, phosphoTyr15. By Western blot the antibody recognizes the ~35 kDa phospho CDC2 protein. The labeling of the CDC2 band in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. |
| Gene Symbol |
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| Modifications |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. SIZE: 297 amino acids; 34095 Da SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. SUBCELLULAR LOCATION: Nucleus (By similarity). SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |