Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, F, Fg | IP, WB, ICC, IHC, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3202 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-LIM-3 Antibody |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LIM-3 Antibody is an antibody against LIM-3 for use in IP, WB, IC, IH, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:250-1:1,000 on paraffin embedded sections. 1:3,000-1:6,000 on frozen sections. 1:4,000-1:8,000 on whole mounts. Recommended fixative MEMFA. Immunocytochemistry: 1:200-1:500 Western blot: 1:2,500-1:5,000 Immunoprecipitation: 1:200 This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | C-terminal portion of mouse LIM-3 protein. |
| Host | Rabbit |
| Specificity | Recognizes LIM-3. This antibody does not appear to cross react with gsh-4 on tissue sections but may cross react in Western blot and immunoprecipitation. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The LIM domain is a double zinc finger structure that promotes protein-protein interactions. LIM domain proteins, such as ABLIM3, play roles in embryonic development, cell lineage determination, and cancer (Krupp et al., 2006 [PubMed 16328021]).[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O94929 # May act as scaffold protein. SIZE: 683 amino acids; 77802 Da SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: SwissProt: O94929 ## Contains 1 HP (headpiece) domain. & Contains 4 LIM zinc-binding domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |