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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3121 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Angiopoietin-2 Antibody, NT |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation. ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | 20 amino acid peptide sequence near the amino-terminus of mouse Ang-2. |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus). The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O15123 # Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. SIZE: 496 amino acids; 56919 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: O15123 ## Contains 1 fibrinogen C-terminal domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |