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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rhesus Macaque | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2971 |
| Description | Anti-LAMP-1 (CD107a) Antibody |
| Alternate Names |
|
| Background Information | Lysosome-associated membrane protein 1 (LAMP-1) is a highly glycosolated, single-pass type 1 membrane protein belonging to the lysosome- associated membrane protein family; a group of proteins that make up the majority of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-1 functions to traffic carbohydrate ligands to selectins. It is also thought to play a role in tumor cell metastasis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-LAMP-1 (CD107a) Antibody is validated for use in WB, IC for the detection of LAMP-1 (CD107a). |
| Key Applications |
|
| Application Notes | Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected LAMP-1 in 10 µg of EL4 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected LAMP-1 in NIH/3T3, A431, and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human LAMP-1 at the topological domain (Lumenal). |
| Epitope | Topological domain (Lumenal) |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes LAMP-1 at the topological domain (Lumenal). |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human, mouse, and rat. Predicted to react with rhesus macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane. PTM: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. SEQUENCE SIMILARITIES: Belongs to the LAMP family. |
| Molecular Weight | ~ 95kDa observed. Although the calculated MW 45 kDa, LAMP-1 is heavily glycosylated and therefore, MW typically can be expected between 90-120 kDa. Uncharacterized bands may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in EL4 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-1 in 10 µg of EL4 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |