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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB, IH(P) | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2910 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Mcl-1 Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Liquid antiserum. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Mcl-1 Antibody is validated for use in IP, WB, IH(P) for the detection of Mcl-1. |
| Key Applications |
|
| Application Notes | Immunoblot 1:2,000 Immunoprecipitation. Precipitates a 37-39 kDa protein, which appears as a doublet in some cells. Immunohistochemistry (formalin-fixed, paraffin-embedded tissues) The following tissues/cells can serve as positive controls: tonsil, tonsillar B cells, B-cell lines Reh, Daudi, and Karpas 422. Optimal working dilutions for AB2910 must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids 121-139 of human Mcl-1. |
| Host | Rabbit |
| Specificity | Recognizes Human Mcl-1. Not reactive with other members of the Bcl-2 family, as verified by immunoblot. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q07820 # Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it. SIZE: 350 amino acids; 37337 Da SUBUNIT: Interacts with BAD, BOK, BIK, BFM and PMAIP (By similarity). Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro- apoptototic BCL2-related proteins. SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential). Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria. PTM: Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. & Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. & Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. SIMILARITY: SwissProt: Q07820 ## Belongs to the Bcl-2 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze-thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |