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| Description | |
|---|---|
| Catalogue Number | AB2302-25UG |
| Description | Anti-GAPDH |
| Alternate Names |
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| Background Information | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a house-keeping enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimers and Huntingtons disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB. |
| Key Applications |
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| Application Notes | Western Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH. |
| Epitope | WARS interacting region |
| Concentration | Please refer to lot specific datasheet. |
| Host | Chicken |
| Specificity | This antibody recognizes GAPDH. |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with human, mouse, and rat. Predicted to react with equine and canine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway. SIZE: 335 amino acids; 36053 Da SUBUNIT: Homotetramer. Binds PRKCI. SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane. SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
| Molecular Weight | ~ 38 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 and PC12 cell lysate. Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |