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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB | Ch | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2302 |
| Description | Anti-GAPDH Antibody |
| Alternate Names |
|
| Background Information | Glyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406; also known as EC: 1.2.1.12, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. GAPDH is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities and is considered as a house-keeping enzyme. It is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. It also plays a role in several nuclear functions. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1 that contains a nuclear localization signal. In the nucleus, it participates in transcription, RNA transport, DNA replication, and apoptosis. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:16,000 dilution from a representative lot detected GAPDH in NIH3T3 cell lysate. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the internal region of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). |
| Epitope | WARS interacting region |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Chicken |
| Specificity | This chicken polyclonal antibody specifically detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). It targets an epitope within 12 amino acids from the internal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Canine, Horse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway. SIZE: 335 amino acids; 36053 Da SUBUNIT: Homotetramer. Binds PRKCI. SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane. SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
| Molecular Weight | Target molecular weight ~38 kDa observed; 36.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:16,000 dilution of this antibody detected GAPDH in HEK293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |