Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Rhesus Macaque | WB, ICC, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2277 |
| Description | Anti-FOXP1 Antibody |
| Alternate Names |
|
| Background Information | Forkhead box protein 1 (FOXP1) is a member of the Forkhead family of transcription factors, which have been characterized as transcriptional repressors and activators, and as having a DNA-binding domain distinguished by its formation of a winged-helix motif. FOXP1 is known to be an important regulator of airway morphogenesis, lung epithelial development, and early cardiac development. It is also known to have an important role in conjunction with NFκβ in the development of B-cells and the differentiation of macrophages. Splice variants give rise to four isoforms of FOXP1. The protein is known to form homodimers and heterodimers with FOXP2 and FOXP4. Studies have indicated FOXP1 as a potential inducer of oncogenesis when over expressed. The depletion and mutation of FOXP1 expression may lead to defects in embryonic cardiac, lung and esophagus epithelial development. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FOXP1 Antibody detects level of FOXP1 & has been published & validated for use in WB, IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:300 dilution of AB2277 from a previous lot detected FOXP1 in inflammatory skin disorder and normal human kidney-glomerulus tissue. Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected FOXP1 in NIH/3T3, RAW264.7, and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of human FOXP1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the C-terminus of FOXP1. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human, mouse, and rat. Predicted to react with bovine, and rhesus monkey based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Transcriptional repressor that plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development (By similarity). SUBUNIT STRUCTURE: Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1 (By similarity). SUBCELLULAR LOCATION: Nucleus Probable. DOMAIN: The leucine-zipper is required for dimerization and transcriptional repression By similarity. INVOLVEMENT IN DISEASE: A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5. SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. SEQUENCE CAUTION: The sequence AAF36135.1 differs from that shown. Reason: Frameshift at positions 531 and 545. |
| Molecular Weight | FOXP1 has 4 isoforms observed at 75/73, 64 and 54 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected multiple isoforms of FOXP1 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |