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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Eq, Gt, H, M, Ht, R, Po, Sh | ELISA, IHC, IP, RIA, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1930 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Integrin alphaV Antibody, CT, Intracellular |
| Alternate Names |
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| Background Information | The integrin family of cell adhesion receptors consists of at least 19 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. To date, 16 different alpha-subunits and 8 different beta-subunits have been identified. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaV is a type I membrane glycoptroein with no I (inserted) domain. The alphaV protein is post-translationally cleaved into a heavy and light chain, which is expressed on the cell surface as a disulfide linked polypeptide. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Neat rabbit antiserum in liquid with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin alphaV Antibody, C-terminus, Intracellular detects level of Integrin alphaV & has been published & validated for use in ELISA, IH, IP, RIA & WB. |
| Key Applications |
|
| Application Notes | ELISA/RIA: A 1:1,000 dilution of a previous lot was used in ELISA/RIA. Immunoprecipitation: 5 μL of a previous lot of this antibody is sufficient to precipitate alphaV/beta1 from 5x106 cells. Immunohistochemistry: 1:1000 dilution from a previous lot was used in immunohistochemistry. (Suggested for use an acetone fixed tissue only.) Western blot: 1:5000. The antibody recognizes a single band at approximately 150 kDa when denatured, but not reduced (Hirsch, 1994). Under reducing conditions this antibody recognizes two major bands of 25 and 27 kDa and, to a lesser extent, the full length 150 kDa band. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alphaV integrin subunit (SwissProt Accession P06756, amino acids 1022-1034). |
| Epitope | C-terminus, intracellular |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Integrin alphaV. Specificity determined by immunoprecipitation from a detergent extract of 35S methonine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, or alpha6 integrin subunits was detected. The mature alphaV protein (150 kDa) is composed of a disulfide bonded heavy (125 kDa) and a light (25 kDa) chain derived from a proteolytically cleaved precursor (Suzuki, 1987). The epitope recognized by the antibody is located on the COOH terminal portion of the light chain. By western blot under reducing conditions the antibody detects two major bands at approximately 25 and 27 kDa corresponding to the light chain. The slight differences in sizes of the light chains is thought to be caused by proteolytic cleavage at two alternative sites on the precursor. Similar sites have been reported for alpha6, alpha3, and alphalIbeta (Hogervorst, 1991). |
| Species Reactivity |
|
| Species Reactivity Note | Chicken, equine, goat, human, hamster, mouse, porcine (pig), and sheep. Expected to react with rat based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions. SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. |
| Molecular Weight | 130 kDa; 25 and 27 kDa (reducing conditions) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on C6 lysates. Western Blot Analysis: 1:500 dilution of this lot detected INTEGRIN AV light chain on 10 μg of C6 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |