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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1797P |
| Replaces | AB3512P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Arginine Vasopressin Receptor V2 Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Arginine Vasopressin Receptor V2 Antibody is an antibody against Arginine Vasopressin Receptor V2 for use in ELISA, IH & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. An antibody raised against this peptide has detected a glycosylated band at ~62 kDa in membranes from rat inner medulla (Nonoguchi et al. 1995). N-Glycanase reduced the size to ~36 kDa (deglycosylated form). Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue. AVP-V2 has been localized in terminal IMCD and other areas of rat kidney (Nonoguchi et al. 1995). ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide to coat plate. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | 21 amino acid peptide from the 3rd cytoplasmic domain of rat AVP-V2 (Lolait et al. 1992; Fisov et al. 1994). |
| Host | Rabbit |
| Specificity | Arginine vasopressin receptor (AVP-V2), rat. The peptide used for immunization has no significant similarity with AVP V1a or V1b receptors. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these extrarenal receptors are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P30518 # Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. SIZE: 371 amino acids; 40279 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Kidney. DISEASE: SwissProt: P30518 # Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD) [MIM:300539]. This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. & Defects in AVPR2 are a cause of X-linked nephrogenic diabetes insipidus, type I (NDI) [MIM:304800]. It is characterized by excessive water drinking (polydypsia) and urine excretion (polyuria) and fail to concentrate urine in response to vasopressin. Most cases of NDI appear to have an X-linked recessive pattern of inheritance. SIMILARITY: SwissProt: P30518 ## Belongs to the G-protein coupled receptor 1 family. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |