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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1726 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Connexin 40 Antibody |
| Alternate Names |
|
| Background Information | Gap junction alpha-5 protein (UniProt: Q01231; also known as Connexin-40, Cx40) is encoded by the Gja5 (also known as Cxn-40) gene (Gene ID: 14613) in murine species. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. Connexins are tetraspan membrane proteins that form interlocking hexamers at each cell membrane to create a pore between two adjacent cells. Connexins usually have a short half-life, which is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. Three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-40 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. Its expression is observed in kidney, heart, and skin; however, it is abundantly present in the lung tissue. It is also recognized as a component of gap junctions in vascular endothelium. It is reported to form channels with a large single channel conductance and moderate voltage-sensitivity. It is widely distributed in developing mouse heart and is expressed in the ventricles and atria at E11, however by day 14 it is restricted to the conduction system in the ventricles, but still, it remains in working atrial myocardium. (Ref.: Verheule, S., and Kaese, S. (2013). Front. Pharmacol. 4; 81; Bruzzone, R., et al. (1993). Mol. Cell Biol. 4(1); 7-20). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Connexin 40, Cat. No. AB1726, is a rabbit polyclonal antibody that detects Connexin 40 and is tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Connexin 40 in lysates from C2C12 cells. Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected Connexin 40 in HepG2 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of mouse Connexin-40. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Connexin-40. It targets an epitope within 15 amino acids from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Human, Mouse, Rat. Predicted to react with Bovine, Canine, Opossum, Non-Human Primates based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P36382 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. SIZE: 358 amino acids; 40380 Da SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. DISEASE: SwissProt: P36382 # Defects in GJA5 are the cause of atrial fibrillation type 4 (ATFB4) [MIM:108770]; also known as atrial standstill. ATFB is characterized by rapid and irregular activation of the atrium. SIMILARITY: SwissProt: P36382 ## Belongs to the connexin family. Alpha-type (group II) subfamily |
| Molecular Weight | ~40 kDa observed; 40.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HUVEC lysate. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Connexin 40 in HUVEC lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |