Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB16982 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Fas Ligand Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Peptide affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Fas Ligand Antibody detects level of Fas Ligand & has been published & validated for use in ELISA & WB. |
| Key Applications |
|
| Application Notes | Western Blotting: 1:1,000-1:5,000 Rat, mouse and human FASL have 278, 279, and 281 AA residues, respectively (calculated MW 32kDa). Western blot of the oocyte has detected a specific 31kDa band (Hakuno et al. 1996). A 40 kDa FASL has been detected in mouse T cells (Hahne 1995). An alternate detecting reagent, mFAS-Fc (Suda & Nagata 1994) also detected a cell surface protein of about 40 kDa. ELISA: 0.5-1μg/mL Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 13 AA peptide sequence mapping near the C-terminus of human FAS ligand (Mita et al. 1994). This peptide is predicted to be extracellular. The peptide was synthesized with C-terminal Cysteine, coupled to KLH. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Human FAS Ligand (FASL). The 13-mer human FASL sequence used for antibody production is 92% (12/13 AA) homologous with rat and mouse FASL. Based upon the sequence homology data, anti-human FASL will likely cross react with the mouse/rat FASL. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). SIZE: 335 amino acids; 37732 Da SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE. SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted. TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |