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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB16527 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-MyD88 Antibody, CT |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MyD88 Antibody, C-terminus is an antibody against MyD88 for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:500 to 1:1000 Whole cell lysate from Jurkat cells can be used as positive control and a 35 kDa band should be detected. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Rabbit anti-MyD88 (C-terminus) polyclonal antibody was raised against a peptide corresponding to amino acids 279 to 296 of human MyD88 (Bonnert et al. 1997), which are identical to those of mouse. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (Muzio et al. 1997). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (Adachi et al. 1998; Medzhitov et al. 1998), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-defecient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q99836 # Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. May be involved in myeloid differentiation. SIZE: 296 amino acids; 33233 Da SUBUNIT: Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1 and IRAK2 via their respective TIR domains. Interacts with IL1RL1. SUBCELLULAR LOCATION: Cytoplasm (Probable). TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: SwissProt: Q99836 ## Contains 1 death domain. & Contains 1 TIR domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |