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| Description | |
|---|---|
| Catalogue Number | AB15708-25UG |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti- beta III Tubulin |
| Alternate Names |
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| Background Information | Tubulin beta-3 chain (UniProt: Q13509; also known as Tubulin beta-4 chain, Tubulin beta-III, beta-Tubulin 3) is encoded by the TUBB3 (also known as TUBB4) gene (Gene ID: 10381) in human. Tubulin is one of several members of a small family of globular proteins, which polymerize to form microtubules. The most common members of the tubulin family are -tubulin and β-tubulin that assemble onto microtubules forming large polymers. Tubulin binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the -chain. GTP-binding site of β-tubulin III is localized to amino acids 140-146. β-tubulin III is a unique microtubule subunit that is expressed exclusively in neurons, but its higher expression is also observed in most cancerous tissues. Due to it is unique expression pattern it is often utilized as a marker to identify neuronal cells from other brain cells such as glia. It plays a critical role in proper axon guidance and maintenance. It undergoes phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase and this phosphorylation inhibits tubulin incorporation into microtubules. Increased expression of b-tubulin III been shown to correlate with resistance to anti-microtubule agents. Mutations in TUBB3 gene have been linked to congenital fibrosis of extraocular muscles that causes ocular motility disorder affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. Some mutations are also known to cause cortical dysplasia that results in aberrant neuronal migration and disturbed axonal guidance. (Ref.: Poirier, K.., et al. (2010). Hum. Mol. Gen. 19(22); 4462-4473; Tischfield, MA., et al. (2010). Cell. 140(1); 74-87; Katsetos, CD., et al. (2003). J. Child. Neurol. 18(12); 851-866). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-β III Tubulin, Cat. No. AB15708, is a rabbit polyclonal antibody that detects β III Tubulin and is tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected β III Tubulin in Rat Brain tissue lysate. Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected β III Tubulin in Neuro2a cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region of Human β III Tubulin. |
| Epitope | C-terminal |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects β III tubulin. It targets an epitope within 10 amino acids from the C-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Mouse, Rat. Predicted to react with Monkey, Bovine, Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 450 amino acids; 50433 Da SUBUNIT: Dimer of alpha and beta chains. DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family. |
| Molecular Weight | ~50 kDa observed; 50.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in Mouse brain tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected β III Tubulin in Mouse Brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at +2°C to +8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |