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Millipore This Anti-Adenosine A2a Receptor Antibody Is Validated For Use In Elisa, Wb For The Detection Of Adenosine A2a Receptor. - Mill (Additional S&H or Hazmat Fees May Apply)
This Anti-Adenosine A2a Receptor Antibody is validated for use in ELISA, WB for the detection of Adenosine A2a Receptor.
Key Applications
ELISA
Western Blotting
Applications Not Recommended
Immunohistochemistry
Application Notes
Western blot: 1:1,000-1:5,000 (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Synthetic 30 amino acid peptide corresponding to amino acids 383-412 of the canine adenosine A2a receptor near the C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected in not present in A1, A2b and A3 receptors.
Host
Rabbit
Specificity
Adenosine A2a receptor. Recognizes a band of approximately 45 kDa in human (coronary artery), and rat, porcine and bovine striatal membranes.
Species Reactivity
Bovine
Canine
Human
Pig
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_000675.4
Entrez Gene Summary
This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
Gene Symbol
ADORA2A
ADORA2
RDC8
hA2aR
UniProt Number
P29274
UniProt Summary
FUNCTION: SwissProt: P29274 # Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. SIZE: 412 amino acids; 44707 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P29274 ## Belongs to the G-protein coupled receptor 1 family.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
