Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, R, Sh | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1538 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Dopamine β Hydroxylase Antibody, NT |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody, N-terminus validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting: 1:250-1:1,000. Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain Showed weak staining on both human and monkey. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide, CSAPRESPLPYHIPLDPEG-amide, cloned from human (DbetaH) cDNA (Nagatsu et al. 1990). |
| Epitope | N-terminus |
| Host | Rabbit |
| Specificity | Dopamine Beta Hydroxylase (DbetaH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline. COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit. SIZE: 617 amino acids; 69065 Da SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers. SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential). DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |