Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB15332 |
| Replaces | AB5637 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Nicotinic Acetylcholine Receptor α7 Antibody |
| Background Information | Neuronal acetylcholine receptor subunit alpha-7 (UniProt: P36544; also known as -Nicotinic Receptor Alpha 7, Nicotinic AChR alpha7) is encoded by the CHRNA7 (also known as NACHRA7) gene (Gene ID: 1139) in human. Nicotinic acetylcholine receptors are cation-selective, ligand-gated ion channels that mediate fast neurotransmission. They are distributed at the post-synaptic membrane of muscle cells. A similar neuronal-type ACh receptor is also found in the central nervous system and is involved in a wide range of physiological and pathological functions. They are assembled either as homo-pentamers of 7, 8, and 9 or hetero-pentamers of 2 6 in combination with β2 β4 or 9 with 10 subunits. They regulate the flow of sodium, potassium, and calcium ions across the cell membrane. The binding of ligands to these receptors triggers a tertiary conformational transition of neuronal AChRs among functionally distinct resting, open, and desensitized states. Nicotinic AChR 7 is a multi-pass, homopentameric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-230), four transmembrane domains (aa 231-255; 262-280; 296-317; and 470-490), and one cytoplasmic domain (aa 318-469). (Ref.: Ho, TN., et al. (2020). Front. Neurosci. 14; 609005; Unwin, N. (2013). Q. Rev. Biophys. 46(4); 283-322). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Nicotinic Acetylcholine Receptor 7, Cat. No. AB15332, is a rabbit polyclonal antibody that detects Nicotinic Acetylcholine Receptor 7 and is tested for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal half of human Nicotinic Receptor 7. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Nicotinic Receptor 7. It targets an epitope with 28 amino acids from the C-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P36544 # After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. SIZE: 502 amino acids; 56449 Da SUBUNIT: Homopentamer. Interacts with RIC3; which is required for proper folding and assembly. SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P36544 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family. |
| Molecular Weight | Target molecular weight 52 kDa observed. 56 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Nicotinic Acetylcholine Receptor 7 in Rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |