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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IHC, IP, NEUT, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1431 |
| Replaces | 04-1095 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Interferon-β Antibody |
| Alternate Names |
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| Background Information | Interferon beta is a globular protein consisting of 5 alpha helices with a predicted molecular weight of 20 kDa. Due to glycosylation, however, the observed molecular weight is closer to 25 kDa by SDS-PAGE. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit Serum diluited with PBS. Liquid containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Interferon-β Antibody is validated for use in ELISA, IH, IP, NEUT, WB for the detection of Interferon-β. |
| Key Applications |
|
| Application Notes | Interferon neutralization: 2 x 10e5 neutralization units/mL where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-b) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," in J. Virol., Vol. 37, 755-758]; [Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods of Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for the effective neutralization of human interferon beta. Immunohistochemistry Western blot: 1:5000 Immunoprecipitation ELISA Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human interferon beta expressed in E. coli |
| Host | Rabbit |
| Specificity | Recognizes Human interferon beta. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants. SIZE: 212 amino acids; 23718 Da SUBCELLULAR LOCATION: Secreted. PTM: N- and O-glycosylated. DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS. SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily. |
| Molecular Weight | 20 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 20000 units |