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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB125 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Serotonin Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit antiserum. Liquid with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | 5-Hydroxytryptamine-glutaraldehyde-Poly-lysine |
| Host | Rabbit |
| Specificity | Serotonin (5-Hydroxytryptamine) The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity 5-Hydroxytryptamine-G-PL 1 5-Methoxytryptamine-G-PL 1/25 Tryptamine-G-PL 1/60 5-Hydroxytryptophan-G-PL 1/175 5-Hydroxytryptamine 1/1150 Tryptophan-G-PL 1/3400 5-Methoxytryptophan-G-PL 1/19800 The antisera was also tested for specificity using the free-floating PAP technique on rat raphe nuclei. Abbreviations: (G) Glutaraldehyde (=) Non-reduced conjugate (PL) Poly-lysine |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |