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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10620 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cdc4 Antibody |
| Background Information | The F-box protein family is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. They constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Cdc4 is one member of this family, which is also known as AGO, FBW7, FBX30 or SEL10. CDC4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Cdc4 proteins of amino acid lengths varying from 553 aa to 707 aa in length have been reported. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event.5,6 Cdc4 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. Thus, it may be important for Alzheimers disease. Mutations of the CDC4 gene are detected in ovarian and breast cancer cell lines and the CDC4 gene may also be involved in the pathogenesis of human pancreatic and endometrial cancers. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | This polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cdc4 Antibody is an antibody against Cdc4 for use in WB & IP. |
| Key Applications |
|
| Application Notes | Western Blotting: 1-3 μg/mL Immunoprecipitation: 7 µg/reaction Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10). |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes CDC4. |
| Species Reactivity |
|
| Species Reactivity Note | Reactivity has been confirmed with human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the genes potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q969H0 # Recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Involved in the degradation of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SIZE: 707 amino acids; 79663 Da SUBUNIT: Part of a SCF complex consisting of CUL1, RBX1, SKP1 and FBXW7. Interacts with PSEN1, cyclin E, NOTCH1 NICD, NOTCH4 NICD and SKP1A. SUBCELLULAR LOCATION: Nucleus (By similarity). TISSUE SPECIFICITY: Isoform 1 is widely expressed. Isoform 4 is expressed in brain. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE:SwissProt: Q969H0 # Defects in FBXW7 may be a cause of breast cancer. SIMILARITY: SwissProt: Q969H0 ## Contains 1 F-box domain. & Contains 7 WD repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C in undiluted aliquots for 1 year from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |