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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, B, Ca | WB, IP, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10546 |
| Description | Anti-Bcl-2-associated transcription factor 1 Antibody |
| Alternate Names |
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| Background Information | Bcl-2-associated transcription factor 1 (Btf) is a ubiquitously expressed death-promoting transcriptional repressor. It has been shown to interact with anti-apoptotic proteins related to Bcl-2, DNA, adenovirus E1B 19K, and EMD. Btf has also been shown to be involved in E1A transformation of p53 or E1B 19K, an interaction that implicates Btf as being involved in transcriptional regulation and in promoting apoptosis. Expression of Btf is absent in some cancers, which may suggest a potential role for the protein in tumor suppression. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Bcl-2-associated transcription factor 1 Antibody is validated for use in WB, IP, IH(P) for the detection of Bcl-2-associated transcription factor 1. |
| Key Applications |
|
| Application Notes | Western Blot (Snap i.d.) Analysis: 0.5 µg/mL from a previous lot detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate. Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Bcl-2-associated transcription factor 1 from HeLa cell lysate. Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Bcl-2-associated transcription factor 1 in neuronal and glial cells in normal human brain tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Bcl-2-associated transcription factor 1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human and mouse. Predicted to react with bovine and canine based on 100% sequence homology. Other homologies: Rat (81% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Death-promoting transcriptional repressor. SUBUNIT STRUCTURE: Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICTY: Ubiquitous. SEQUENCE CAUTION: The sequence AAH47687.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH47887.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH56894.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH63846.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA11481.2 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~120 kDa observed. This protein has 4 isoforms: Isoform 1 (106 kDa calculated), Isoform 2 (106 kda calculated), Isoform 3 (100 kDa calculated), Isoform 4 (86 kDa calculated). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Bcl-2-associated transcription factor 1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |