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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10439 |
| Description | Anti-PACSIN1 Antibody, NT |
| Alternate Names |
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| Background Information | PACSINs, also known as syndapins, are a protein family consisting of three members that have been implicated in synaptic vesicular membrane trafficking and regulation of dynamin-mediated endocytotic processes (Cuajungco, 2006). It is presumed that all isoforms bind Dynamin, Synaptojanin 1 and N-WASP through their Src homology 3 domains. While PACSIN1 appears to be restricted to neural tissues, two PACSINs are more widespread with PACSIN2 being ubiquitously expressed and PACSIN3 present mainly in skeletal muscle, heart and lung (Modregger, 2001). PACSIN1 has been implicated as playing a central role in synaptic vesicle recycling (Kessels, 2004). Expected molecular weight is 51 kDa. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | PBS containing 0.09% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PACSIN1 Antibody, N-terminus detects level of PACSIN1 & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH conjugated synthetic peptide selected from the N-terminal region of human PACSIN1. |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes human PACSIN1. |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Reactivity with other species has not been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: May play a role in vesicle formation and transport. Ref.6 SUBUNIT STRUCTURE: Homo- and hetero-aggregates with other PACSINs. Binds dynamin I, synaptojanin, synapsin I and the neural Wiskott-Aldrich syndrome protein (N-WASP) By similarity. SUBCELLULAR LOCATION: CytoplasmBy similarity. Note= Colocalizes with dynamin I at vesicular structures in the cell body and neurites By similarity. TISSUE SPECIFICITY: Highly expressed in brain and in a lesser extent in heart, pancreas and liver. PTM: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable. SEQUENCE SIMILARITIES: Belongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain. |
| Molecular Weight | ~57 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated on a representative lot by Western blot on mouse brain lysate using a 1:100 - 1:500 dilution of Anti-PACSIN1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8ºC for up to 6 months from date of receipt. -20ºC is recommended for long term storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |