| Description | |
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| Catalogue Number | 21-169 |
| Brand Family | Upstate |
| Trade Name |
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| Description | FOPflash (mutant TCF binding sites) |
| Product Information | |
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| Quality Level | MQ100 |
| Applications | |
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| Application | Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negative control to TOPflash. |
| Key Applications |
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| Biological Information | |
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| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box (E-box) binding site (CANNTG) - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5-GTTAATNATTAAC-3. SIZE: 631 amino acids; 67356 Da SUBUNIT: Binds DNA as a dimer. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Liver. DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]. SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Packaging Information | |
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| Material Size | 5 µg |