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Key Specifications Table
| Species Reactivity | Key Applications |
|---|---|
| H, M, R | WB, ChIP |
| Description | |
|---|---|
| Catalogue Number | 17-601 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set |
| Overview | Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin. The qPCR primers included flank the Sp1 binding site in human DHFR promoter. |
| Alternate Names |
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| Background Information | Transcription factor SP1 can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Anti-Sp1 (Rabbit polyclonal IgG). 1 vial containing 100 μg of protein A purified IgG in 100 μL of 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C. The Sp1 antibody is made against full length human Sp1 protein and can recognize Sp1, MW 105 kDa and 95 kDa, of human, mouse and rat origins. Normal Rabbit IgG. One vial containing 125 μg of normal rabbit IgG in 125 μL. Store at -20°C. ChIP primers DHFR. 1 vial containing 75 μL of 5 μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C. FOR: TCG CCT GCA CAA ATA GGG AC REV: AGA ACG CGC GGT CAA GTT T |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | SP1 ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of SP1. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures). |
| Biological Information | |
|---|---|
| Immunogen | Full length human Sp1 protein |
| Host | Rabbit |
| Specificity | Sp1 |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter. SIZE: 785 amino acids; 80693 Da SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. SUBCELLULAR LOCATION: Nucleus. PTM: O-glycosylated; contains N-acetylglucosamine side chains. SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers. |
| Molecular Weight | 105 kDa and 95 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Chromatin immunoprecipitation: Sonicated chromatin prepared from 1 x 106 HeLa cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPTM A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR flanking the human DHFR promoter that contains a Sp1 binding site (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat.# 17-408) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 25 assays |
| Material Package | 25 assays per kit, ~4μg per chromatin immunoprecipitation. |